HiPure Fastfilter Plasmid Maxi Kit

Introduction The HiPure FastFilter Plasmid DNA Maxi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes a special filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 1000 µg high copy number plasmid DNA or 50-500 µg low copy number plasmid DNA can be purified from 200mL overnight culture. Details Specifications Features Specifications Main Functions Isolation up to 1mg plasmid DNA from 200ml bacterial culture Applications Enzyme digestion, sequencing, PCR and labeling,  etc. Purification method Maxi spin column Purification technology Silica technology Process method Manual (centrifugation or vacuum) Sample type Conventional plasmid, plasmid less than 30KB Sample amount 100-200ml Yield 0.4-1mg Elution volume ≥500μl Time per run ≤60 minutes Liquid carrying volume per column 20ml Binding yield of column 1mg Principle The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA). Advantages High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc. Fast – it takes only 15-60 minutes to complete the isolation Economy – high cost performance Kit Contents Contents P101402 P101403 Purification Times 10 Preps 50 Preps RNase A 20 mg 40 mg Buffer P1 140 ml 2 x 350 ml Buffer P2 140 ml 2 x 350 ml Buffer LEN3 70 ml 350 ml Buffer GBT 120 ml 550 ml Buffer PW1 60 ml 300 ml Buffer PW2* 50 ml 4 x 100 ml Elution Buffer 20 ml 120 ml HiPure DNA Maxi Columns III 10 50 Lysate Clear Maxi Syringe 10 50 50 ml Collection Tubes 20 100 Storage and Stability The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C. Purchase Guide Name CAT NO Sample amount Column type Elutio volume Yield Time per run Centrifuge requirements HiPure Plasmid Mini Kit P1001 1-5ml 1.5ml column 30-50μl 5–35µg/5ml ≤30 minutes small centrifuge HiPure Plasmid Mini Kit II P1002 5-15ml 1.5ml column 75-100μl 20-80µg/15ml ≤30 minutes small centrifuge HiPure Plasmid Plus 96 Kit P1006 1-1.5ml 96 well plate 70-150μl 1-15μg/1ml ≤60 minutes 96 well plate centrifuge HiPure Fastfilter Plasmid Midi Kit P1013 30-50ml 15ml column 0.3-0.8ml 50–250µg/50ml ≤60 minutes 15ml centrifuge HiPure Fastfilter Plasmid Maxi Kit P1014 100-200ml 50ml column 0.5-1.5ml 0.4–1mg/200ml ≤60 minutes 50ml centrifuge